Skip to main content
. 2021 Nov 15;113(1):79–90. doi: 10.1111/cas.15177

FIGURE 1.

FIGURE 1

A, circRNA expression profiles between H1975OR and H1975 cells, the top 5 upregulated circRNAs were identified. B, qRT‐PCR analysis of hsa_circ_0005576 expression in H1975/H1975OR and HCC827/HCC827OR cells. C, Schematic diagram illustrates the genomic location (CDC42) and the backsplice conformation of hsa_circ_0005576. Red and blue arrows represent the fragment of divergent primers specific for backsplice junction of hsa_circ_0005576. D, Gel electrophoresis of hsa_circ_0005576 amplified by divergent primers in total cDNA and gDNA. Sanger sequencing validates the backsplice junction of hsa_circ_0005576. E, qRT‐PCR analysis of hsa_circ_0005576 and CDC42 mRNA expression in H1975OR and HCC827OR cells after treatment with RNase R. F, G, Actinomycin D assay for the abundance of CDC42 mRNA and hsa_circ_0005576 in H1975OR and HCC827OR cells. *P < .05