Skip to main content
. 2021 Nov 15;113(1):79–90. doi: 10.1111/cas.15177

FIGURE 3.

FIGURE 3

A, qRT‐PCR analysis for abundance of the candidate miRNAs pulled down by biotin‐labeled hsa_circ_0005576 in H1975OR cells. B, qRT‐PCR analysis measures the expression changes of candidate miRNAs in H1975OR cells transfected with or without sh‐circ_0005576. C, Predicted binding sites between miR‐512‐5p and hsa_circ_0005576. Construction of wild‐type (WT) and mutant (MUT) hsa_circ_0005576 dual‐luciferase reporter vectors. D, Dual‐luciferase activity of WT hsa_circ_0005576 3′UTR or mutant hsa_circ_0005576 3′UTR after transfection with miR‐512‐5p mimics in H1975OR cells. E, Colony forming assay evaluates cell proliferation in LUAD transfected with or without miR‐512‐5p mimics, then treated with osimertinib (1 μM). *P < .05