A, Distribution of tumor‐associated macrophages (TAMs) and FOXP3‐positive regulatory T lymphocytes (Tregs) in diffuse large B‐cell lymphoma (DLBCL). The frequencies of different markers of macrophages (TAMs) and FOXP3+ Tregs varied between samples. The following markers were examined: CD68 (pan‐macrophages), CD16 (pro‐inflammatory, M1‐like, with cytotoxic and antitumoral properties), CD163, pentraxin 3 (PTX3), microphthalmia transcription factor (MITF), and interleukin‐10 (IL‐10) (protumoral M2‐like; PTX3 and IL‐10 for the immune regulatory M2c‐like subtype). Original magnification, ×200 (Olympus BX63). B, Histological features of TAMs and FOXP3‐positive Tregs in DLBCL. This figure shows the immunohistochemical expression of different markers and histological characteristics of TAMs and Tregs, focusing on DLBCL cases with a high infiltration of these cells in the immune microenvironment. The effects of and relationship between the different cells in the microenvironment in DLBCL are also shown; red indicates inhibition, green indicates activation. Original magnification, ×200 (Olympus BX53)