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. Author manuscript; available in PMC: 2022 Jan 11.
Published in final edited form as: Mol Plant Microbe Interact. 2021 Dec 7;34(12):1433–1445. doi: 10.1094/MPMI-07-21-0163-R

Figure 3. RT-PCR to detect alternative splice forms of Glyma.15G191200.

Figure 3.

(A) Predicted intron 5 retention transcript model based on published RNAseq data (Phytozome). (B) RT-PCR using primers specific to alternative splice form (arrows in A) detected the intron 5 retention splice form in all tested contexts: resistant (LD10-30110), susceptible (LD10-30092), with or without SCN infection. No reverse transcriptase (No RT) controls test that amplification is from cDNA and not background genomic DNA. GmSKP16 serves as internal control for cDNA quality and abundance (C) Transcript models for primary fully spliced transcript and additional splice forms revealed upon cloning of the intron retention splice form shown in (A) carrying an intron retention and/or exon exclusion. SNPs unique to cqSCN-006 indicated on gene models. The locations of the premature stop codons are indicated with a red asterisk.