(A, C, E, G) Densitometry analysis of SNAP protein levels from the indicated 2 dpi or 5 dpi time point. n = 12 samples for each treatment (3 each from four independent biological replicate experiments). Mean and standard error of mean are for all data points normalized to mean for susceptible mock within each experiment. Means of treatments with the same letter are not significantly different (P > 0.01 for all except P > 0.05 for G) ANOVA Tukey, analysis performed on non-normalized data). (B, D, F, H) Representative SNAP immunoblots for each time point. Ponceau S stain tests for equal loading across samples. For ɣ-SNAP blots, the lower band that aligns with the predicted size of ɣ-SNAP was measured.