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. 2021 Nov 15;96(1):321–334. doi: 10.1007/s00204-021-03186-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Effects of parathion, paraoxon, malathion and malaoxon on viability, cell death and protein content. PCLS were exposed for 24 h to different concentrations of parathion (A), paraoxon (B), malathion (C) or malaoxon (D) (10–2000 µmol/L). Viability was analyzed by Alamar Blue assay and effects on cellular death were evaluated by measurement of LDH release into PCLS supernatant. For detection of severe tissue destruction, intracellular protein content was detected by BCA assay (E). Data are presented as % of control (Alamar Blue assay and protein content) or as % of the positive control Triton-X (LDH). Data are shown as mean ± SEM. Asterisk indicate significant differences to control (*p < 0.05; **p < 0.01; ***p < 0.001; n = 6 PCLS from three different animals)