Fig. 4.

IRAK3 mediates the effects of miR-539-5p on inflammatory injury of septic H9c2 cells. A Western blot assays were performed to evaluate theIRAK3 expression in H9c2 cells after transfection with pc-IRAK3.^**P < 0.01 vs. pc-DNA3.1 group. ELISA assay was used to determine the expressions of B TNF-α, C IL-1β and D IL-6 in LPS-induced H9c2 cells after transfection. E The viability of LPS-induced H9c2 cells after transfection was assessed by CCK-8 assay. F The proliferation of LPS-induced H9c2 cells after transfection was evaluated by EdU assay. G The expression levels of proteins, including PCNA and Ki-67, were evaluated by western blot assay. H The apoptosis of LPS-induced H9c2 cells after transfection was evaluated by TUNEL assay. I The expression levels of proteins, including Bcl-2, Bax, cleaved caspase-3 and cleaved caspase-9, were evaluated by western blot assay. ^*P < 0.05, ^**P < 0.01 vs. miR-NC group, ^#P < 0.05, ^##P < 0.01 vs. miR-539-5p mimic group. All data were presented as mean ± SD. n = 3