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. 2022 Jan 10;13:81. doi: 10.1038/s41467-021-27622-9

Fig. 3. Modulation of cardiomyocyte apoptosis by anti-BMP1.3 antibody.

Fig. 3

a Representative images of TUNEL and α-actinin staining on heart sections of mice untreated (sham) or injured by a MI in the absence of treatment (control) or after injection of anti-BMP1.3 antibody (150 µg/kg). Nuclei were stained with DAPI. b Quantification of dying cells in the infarcted area. c Quantification of TUNEL+ nuclei in primary cardiomyocytes and cardiac fibroblasts. d Representative images of primary cardiac cells, either untreated (control) or treated with anti-BMP1.3 antibody (5 µg/ml), stained with TUNEL and anti-α-actinin antibody. Nuclei were stained with DAPI. e Representative images of primary cardiac fibroblasts and cardiomyocytes, cultured in hypoxic conditions, either in the absence (control) or in the presence of recombinant BMP1.3 (0.1 µg/ml), stained with TUNEL and anti-α-actinin antibody. Nuclei were stained with DAPI. f Quantification of TUNEL+ nuclei in cardiomyocytes and cardiac fibroblasts. Data in b, c, and f are shown as mean ± s.e.m. Sample number is indicated inside or above each bar. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparison test in b, c and f. Scale bars in a, d and e indicate 100 µm. Source data are provided as a Source Data file.