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. 2022 Jan 10;13:107. doi: 10.1038/s41467-021-27732-4

Fig. 1. GRH-1 is a longevity-promoting transcription factor.

Fig. 1

a Lifespan assay of wild-type (WT) N2 nematodes on post-developmental grh-1 RNAi compared to control vector L4440. b Lifespan assay on OP50 bacteria using a grh-1 deletion strain (null mutant VC2072) vs. WT (N2). c–e WT (N2) vs. grh-1 OEx line 1 (without heat shock) RT-qPCR of grh-1 with n = 8 for both (c), microscopy images to detect GRH-1::GFP (d), and lifespan on OP50 bacteria (e). f–h Same experiments as in (c–e) but in each case using heat shocked grh-1 OEx line 1 vs. respective WT (N2) control (RT-qPCR with n = 7 for both). i, j Motility (i) and fertility (j) assays of WT (N2) vs. grh-1 OEx sans (−HS) or with (+HS) heat shock (motility assay with n = 3 for all; fertility assay with n = 9 for −HS and n = 10 for +HS). k Western blot of phospho-p38 MAPK in WT (N2) and grh-1 OEx with α-tubulin as loading control. l, m Lifespan assay of WT (N2) vs. Δgrh-1 and grh-1 OEx on pathogenic M. nematophilum (l) or P. carotovorum bacteria (m). Data in bar graphs are mean ± SEM, with sample sizes as stated and individual data points representing biological replicates. P-values were determined with two-tailed unequal variances t-tests of the indicated comparisons of unpaired control vs. treatment groups. P-values of C. elegans lifespan assays were determined by log-rank test. See Supplementary Data 1 for detailed lifespan assay statistics, Supplementary Fig. 1a, d for microscopy images corresponding to (d and g), and Supplementary Fig. 2f for full western blot images corresponding to (k). Source data are provided as a Source data file.