Figure 4.
Antitumor effect of inhibitors on miPS-CSC xenografts. (a) Isolated xenograft tumors from different groups of mice after treated with inhibitors. BALB/c nude mice were challenged subcutaneously with 1 × 106 miPS-LLCcm cells for 9 days, followed by administration 0.1% Tween 80, Gefitinib (100 mg/kg, QOD), Eganelisib (5 mg/kg, QOD), or combination of Gefitinib plus Eganelisib (7 mice per group) for another 12 days. Ruler scale is in cm. (b) Measurement of tumor volume and (c) weight from different groups of mice after treated with inhibitors. (d) Representative H&E staining of developed tumors treated with or without inhibitors. (e) Immunostaining against Ki-67 in tumors from mice treated with or without inhibitors. (f) TUNEL assay of tumor from mice treated with or without inhibitors. Original magnification for H&E, Ki-67 and TUNEL staining is × 100. (b,c) Data were statistically analyzed by Tukey’s multiple comparisons test and expressed as mean ± s.d., where ***P < 0.001.