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. 2022 Jan 10;8:8. doi: 10.1038/s41420-021-00800-w

Fig. 4. Chronic low-dose MET administration restored the autophagic flux to attenuate the senescence of lens epithelial cells in naturally aged mice.

Fig. 4

a Relative fold-changes in the mRNA levels of the genes encoding P62 as determined by qRT-PCR. b Representative images from IHC assays against P62 in the Young, the Aged and the MET. c Representative images from IHC assays against LC3 in the Young, the Aged and the MET. d Western blot analysis of AMPK, p-AMPK, ACC, p-ACC and β-actin in the Young, the Aged and the MET. Data were shown as mean ± SD and are representative of 3 independent experiments. **p < 0.01; ***p < 0.001 compared to the Aged. The bar represents 20 μm.