Fig. 5. NRMT inhibits chemosensitivity of WERI-RB-1/CDDP cells through Bcl2.

A The interaction among NRMT, CENPA, Myc, and Bcl2 predicted by String. The redder the line between genes, the stronger the interaction, and the whiter the weaker. B The silencing efficiency of Bcl2 determined by RT-qPCR (*p < 0.05 vs. cells transfected with sh-NC). C The expression of Bcl2 determined by RT-qPCR. D The expression of Bcl2 determined by western blot analysis (*p < 0.05 vs. cells transfected with oe-NC). E Cell viability assessed by CCK8 assay. F IC₅₀ value determined by CCK8 assay. G Cell proliferation assessed by EdU assay. H Cell apoptosis assessed by flow cytometry. I Expression of PARP, Cleaved PARP, caspase 3, and Cleaved caspase 3 in cells after transfection determined by western blot analysis. *p < 0.05 vs. cells transfected with oe-NC; ^#p < 0.05 vs. cells co-transfected with oe-NRMT + sh-NC. *^/#p < 0.05; **^/##p < 0.01; ***^/###p < 0.001; ****^/####p < 0.0001. The experiment was independently repeated three times.