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. 2022 Jan 10;8:10. doi: 10.1038/s41420-021-00810-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Representative blots and the results showing membrane recruitment of calpain 1 (CAPN1) and the fragments of α-spectrin. NHE and GAPDH were used as the loading controls for membrane proteins and total proteins, respectively. B Plasma levels of LDH and cTnI. C The results of caspase-3 activity and representative blots and densitometric analysis of PARP-1 with GAPDH as an internal control. D The results showing heart function and mean arterial pressure (MAP). Each independent data with the mean is presented. n = 6 rats in each group. Western blotting was performed in six independent biological experiments, and there were three technical replicates per sample. *P < 0.05, **P < 0.01 vs. the burn group. M + Burn refers to rats that received rhTNFR:Fc 30 min post-burn.