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. 2022 Jan 10;8:2. doi: 10.1038/s41420-021-00618-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The expression of AKT1 and Twist1 in periosteal tissues determined by western blot analysis. B IHC staining analysis of STRO-1 protein in periosteal tissues (scale bar = 25 μm) and the quantitative analysis. C Periosteum calcified nodule staining (scale bar = 50 μm). D ALP activity in periosteal tissues. E OCN content in periosteal tissues. *p < 0.05 compared with rats receiving the Masquelet-induced membrane technique treated with inhibitor-NC, ^#p < 0.05 compared with rats receiving the Masquelet-induced membrane technique treated with miR-149-inhibitor + vector. Data were expressed as mean ± standard deviation. Comparison among multiple groups was conducted by one-way ANOVA, followed by Tukey’s post hoc test. MI the Masquelet-induced membrane technique, NC negative control, IHC immunohistochemistry, STRO-1 stromal cell antigen 1, ALP alkaline phosphatase, OCN osteocalcin, ANOVA one-way analysis of variance.