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. 2022 Jan 10;8:17. doi: 10.1038/s41420-021-00794-5

Fig. 5. EVs-miR-142-5p blocks HGF/c-Met and EGF/EGFR pathways by targeting HGF and EGF.

Fig. 5

A KEGG pathway enrichment analysis of downstream target genes of miR-142-5p (The abscissa was generation, the ordinate was KEGG function item, and the histogram on the right was color scale). B Interaction network analysis of enriched candidate target genes in PI3K-AKT signaling pathway (Each circle in the graph represented a gene, and the lines between circles indicated that there was interaction between genes. The more interaction genes existed in a gene, the higher the degree value and the higher the core degree). C Statistics of degree value of core gene (Horizontal coordinate represented degree value, and ordinate represented gene name). D Prediction of miR-142-5p binding sites to EGF and HGF by TargetScan database. E Dual-luciferase reporter assay verifying the targeting relationship of miR-142-5p with HGF and EGF. F The protein expression of HGF, p-c-Met, EGF, and p-EGFR in 5-8FR cells lines detected by western blot. *p < 0.05. All experiments were repeated three times.