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. 2022 Jan 10;13:169. doi: 10.1038/s41467-021-27798-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Intensity imaging showing nuclei labeled with DAPI. b Intensity image showing Tubulin protein labeled with Alexa488. c Intensity image showing Vimentin protein labeled with TRITC. d Intensity image at 647 nm showing mRNA targets, POLR2A and MTOR, which were further resolved by lifetime. e Unmixed lifetime image showing POLR2A puncta labeled with Alexa647. f Unmixed lifetime image showing mTOR puncta labeled with ATTO647. g Merged image of all channels. Scale bar is 10 µm. h Signal-to noise and puncta count analysis for the mRNA targets. Overlaid lines correspond to quantiles [10,50,90]%, n=1757 and n=681 transcripts respectively. Source data are provided as a Source Data file.