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. 2022 Jan 10;8:2. doi: 10.1038/s41523-021-00370-1

Fig. 2. ER-stress down-regulated AR mRNA expression through transcriptional level in LAR TNBC and PCa cells.

Fig. 2

a, b Breast cancer cell lines MDA-MB-453, CAL-148, MFM-223 and HCC2185 (a), and prostate cancer cell lines LNCap and C4-2 (b) were treated with 1 μM TG or 5 μg/mL BFA for 24 h. Then cells were collected for quantitative RT-PCR assay. The relative expression of AR was normalized to β-actin and expressed as mean ± SD (n = 3). Student’s t-test, **p < 0.01 vs. control. c, d MDA-MB-453 (c) and CAL-148 (d) cells were pretreated with 100 μg/mL CHX for 1 h and then treated with or without 1 μM TG during the indicated times. After TG treatment, cells were collected for western blotting assays. e LNCap cells were transfected with empty vector (pMG) or overexpression of AR vector (pMG-AR) and then treated with or without TG during the indicated times. Cells were collected for western blotting assays. f, g MDA-MB-453 (f) and LNCap (g) cells were pretreated with 1 μg/mL ActD for 0.5 h and then treated with or without 1 μM TG during the indicated time. Then, cells were collected for quantitative RT-PCR assays (n = 3). Thapsigargin, cycloheximide, actinomycin D is abbreviated as TG, CHX, and ActD, respectively.