Southern blot of chromosomal DNA of different M. bovis isolates showing hybridization with the labeled 500-bp fragment. Lane 1, strain 868-30; lane 2, strain 621-1; lane 3, strain 621-2; lane 4, strain 621-10; lane 5, strain 621-11; lane 6, strain 621-13; lane 7, strain 621-15; lane 8, strain 621-16; lane 9, M. bovis ATCC 27290; lane M, molecular marker consisting of a 100-bp ladder; λ, molecular marker consisting of HindIII-digested DNA.