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Agarose gel electrophoresis (Metaphor, 1.8%) of amplified DNA of the hsp65 gene digested with HaeIII and BstEII. Lane M, molecular marker V (Boehringer Mannheim, Monza, Italy); lane 1, strain 868-30; lane 2, strain 621-1; lane 3, strain 621-2; lane 4, strain 621-10; lane 5, strain 621-11; lane 6, M. bovis ATCC 27290; lane 7, M. avium as a negative control.
