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. 2022 Jan 10;12:380. doi: 10.1038/s41598-021-04346-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The overall workflow of the study. Animals were bred at three project sites: Site #1: Legacy Research, Site #2: Trinity College, and Site #3: the University of Alabama at Birmingham (UAB). Hippocampus was harvested from each animal and sent to the University of North Dakota for sequencing analysis. RRBS: reduced representation bisulfite sequencing. The workflow was created using Adobe Illustrator with the rat and hippocampus images obtained from http://en.wikimedia.org under the Creative Commons Attribution-ShareAlike 3.0 license.