Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 10;13:181. doi: 10.1038/s41467-021-27801-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Schematic overview of the study design and number of samples per clinical group. b Uniform Manifold Approximation and Projection (UMAP) embedding of the entire dataset consisting of 174,962 cells. The cells are colored by orthogonally generated clusters, and labeled by manual cell type annotation (HE-Fibro: healing enriched fibroblasts, Fibro: fibroblasts, SMCs: smooth muscle cells, BasalKera: basal keratinocytes, DiffKera: differentiated keratinocytes, Sweat/Seba: sweat and sebaceous gland cells; Melano/Schwann: melanocytes and Schwann cells; Mast: mast cells; VasEndo: vascular endothelial cells; LymphEndo: lymphatic endothelial cells; CD14-Mono: CD14+ monocytes, CD16-Mono: CD16+ monocytes, M1-Macro: M1 macrophages, M2-Macro: M2 macrophages, Erythro: erythrocytes, NK: natural killer cells, T-Lympho: T-lymphocytes, NKT: NK cells and T lymphocytes; B-Lympho: B-lymphocytes, Plasma: plasma cells, DCs: dendritic cells). Dotted lines are drawn around cell groups of similar lineages. c Dot plot showing expression of different cell type-specific marker genes, used to annotate the cell types. Size of dots indicates percentage of cells in each cell cluster expressing the marker gene; color represents averaged scaled expression levels; cyan: low, red: high. d Stacked bar plots showing the proportion of different cell types across the four clinical groups. Green: Healthy subjects, orange: DFU-Healers, red: DFU-Non-healers, purple: Diabetic patients. Cell types with significant differences among the clinical groups are marked with an asterisk. The bar plots for individual cell types are presented in Supplementary Fig. 1. e Heatmap showing the top highly expressed (red) genes in each of the cell clusters. f Feature plots depicting the expression of key genes (I) MMP1, (II) MMP3, (III) CHI3L1, (IV) TNFAIP6, that were significantly overexpressed in the healing enriched fibroblasts associated with healing of DFUs. The schematic on (a) was created with BioRender (BioRender.com).