Figure 2.

Effects of diurnal phase on anxiety tests. (A) There is no significant difference in distance traveled or time spent in the center zone of open field between active and inactive groups in the initial test, nor in the retest 3 months later. (B) In the elevated-O-maze, there is no significant difference detected in distance travel or times spent in the open arms between active and inactive groups. However, 3 months later, the mice from the inactive group travelled a higher distance, but with no significant difference in time spent in the open arms in the retest. (C) In the light–dark box, there is no significant difference in time spent in the light box in the initial test. In the retest 3 months later, mice tested in active phase spent less time in the light compartment. Blue circles: male mice; Red circles: female mice.