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. 2022 Jan 10;13(1):35. doi: 10.1038/s41419-021-04491-0

Fig. 4. FOXO3a is a key factor in sorafenib-induced apoptosis.

Fig. 4

A Western blot analysis of the relative expression levels of FOXO3a (90 kDa) in HCC cells transfected with si-FOXO3a or FOXO3a-TM plasmid. B Densitometric analysis of FOXO3a/β-Actin ratios (t-test). C Western blot analysis of pro-caspase-3 (35 kDa) and cleaved-caspased-3 (19, 17 kDa) expression in HCC cells (cultured with sorafenib10 µM + NC, sorafenib 10 µM + FOXO3a-TM, sorafenib 10 µM + si-FOXO3a). D Flow cytometric analysis of cell apoptosis rate in HCC cells cultured with sorafenib10 µM + NC, sorafenib 10 µM + FOXO3a-TM, or sorafenib 10 µM + si-FOXO3a (t-test). Data represent mean ± SD (n = ≥3 experiments). *P < 0.05, **P < 0.01, ***P < 0.001.