Skip to main content
. 2022 Jan 10;13:97. doi: 10.1038/s41467-021-27658-x

Fig. 5. The CXCR3 pathway mediates increased TIL infiltration in GPR182−/− mice.

Fig. 5

a–c 16 days after tumor inoculation, YUMM1.7 tumors in WT and GPR182−/− mice were collected for analyses. Intratumoral chemokine concentrations in YUMM1.7 tumors were measured using LEGENDplex ProInflammatory Chemokine Panel (a). WT, n = 6; GPR182−/−, n = 8. The densities of CXCR3- and CXCR3+ cells in CD8+ (b) and CD4+ (c) T cells within YUMM1.7 tumors were quantified by flow cytometry. WT, n = 7, GPR182−/−, n = 9. Representative data from two independent experiments. d–f Tumor growth curves of WT and GPR182−/− mice that were inoculated with 50,000 YUMM1.7 tumor cells and were followed with or without the treatment of CXCR3 mAb (d). Flow cytometric quantification of CD8+ (e) and CD4+ T cell (f) densities in WT and GPR182−/− mice 16 days after tumor inoculation. n = 10 per group. Representative data from two independent experiments. P-values from two-sided Students t-test (a), one-way ANOVA with Bonferonni correction for multiple comparisons (b, c, e, f), and two-way ANOVA test (d). Error bars represent SEM.