Fig. 7. GPR182 promiscuously binds chemokines via the GAG-binding motif.

a A panel of 35 human chemokines were tested for their ability to block the binding GPR182 + CHO cells by hCXCL9-AF488. % blocking is shown for all chemokines, with chemokines that blocked strongly shown in black bars (>90% blocking), moderately in dark gray (80–90% blocking), and weakly in light gray (<80% blocking). Representative data from two independent experiments. b WT and GPR182 + CHO cells were stained for hCXCL9-AF488 binding at 4 degree. Cells were pre-incubated with a 25-aa peptide of CXCL9 (CXCL9(69-93)) to assess its blocking capacity. Representative data from at least three independent experiments. c Two synthetic GAG-binding peptides were assessed for their ability to block the binding of GPR182 + CHO cells by CXCL9-AF488. Representative data from two independent experiments.