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. 2022 Jan 10;7:1. doi: 10.1038/s41541-021-00424-2

Fig. 5. Vaccination with MultiTEP-based DNA vaccines significantly ameliorated the loss of neurons in hα-Syn Tg mice.

Fig. 5

a Representative images of male mice of immunostained with the pan-neuronal marker-NeuN. Each panel illustrates from top to bottom an overview of the brain, neocortex, hippocampus, striatum, and substantia nigra regions in non-Tg, and αSyn Tg mouse treated with vehicle, PV-1947D, PV-1948D, PV-1949D, and PV-1950D vaccination. b Image analysis of NeuN-positive neuronal cell count per 102 in the neocortex, hippocampus, striatum, and substantia nigra of male mice. c Representative images of female mice immunostained with the pan-neuronal marker-NeuN. Each panel illustrates from top to bottom an overview of the brain, neocortex, hippocampus, striatum, and substantia nigra regions in non-Tg, and αSyn Tg mice with the vehicle, PV-1947D, PV-1948D, PV-1949D, and PV-1950D vaccination. d Image analysis of NeuN-positive neuronal cell counts per 102 in the neocortex, hippocampus, striatum, and substantia nigra in female mice. The scale bar in the overview is 250 µm, the others are 25 µm. *p < 0.05, **p < 0.01, ***p < 0.001.