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. 2022 Jan 10;13:39. doi: 10.1038/s41467-021-27804-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The anchoring of 1 into the hydrophobic binding pocket of albumin (Alb) leads to the creation of Alb-Ru, which is capable of performing ring-closing metathesis. b Reactivity studies focused on the RCM activity of precursor 12 under various substrate concentrations and solvents. ^a20 mol% of glutathione added. Reactions were performed in triplicate; TON values were determined from product yields obtained by HPLC analysis. Michaelis–Menten kinetic graphs c and parameters d for substrates (2, 12, 14, 15). Summarized values are the substrate affinity (K_M), turnover frequency (k_cat), and catalytic efficiency (k_cat/K_M). Also presented are the TON_Arom using 1 mM substrate concentrations, which were incubated with 1 mol% of Alb-Ru in PBS/1,4-dioxane (9:1) for 2 h at 37 °C. Reactions were performed in triplicate. Data in c are represented as mean values ± SD, n = 3 independent experiments.