Fig. 5. In cellulo prodrug therapy against cancer cell growth.
a Summary of calculated GR50 values for drug 20, prodrug 17 and prodrug/Alb-Ru mixtures against HeLa, A549, PC3 and MCF7 cell lines. GR50 values represent concentrations that gives half maximal growth rate inhibition. b Representative dose response curves against HeLa cells showing the cytostatic activities of drug 20, prodrug 17, and mixtures of Alb-Ru (0.5 μM) with prodrug 17. c Schematic depiction of HeLa targeted activation of prodrug 17 into drug 20 using GArM-Ru. The inhibition of HeLa cell growth was investigated using varying mixtures of GArM-Ru and prodrug 17. In general, the prodrug (and drug) was kept at constant concentrations of 4 μM, while varying concentrations of GArM-Ru was used. Data in (b) and (c) are represented as mean values ± SD, n = 3 biologically independent samples.