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. 2022 Jan 10;13:114. doi: 10.1038/s41467-021-27684-9

Fig. 4. Altered Acp5 expression affects the levels of β-catenin.

Fig. 4

a–d Western blot analysis of the levels of β-catenin in WT or Acp5−/− PMLFs (a, p = 0.0239), ACP5 siRNA or Scrambled siRNA treated PHLFs (b, p = 0.0034), Acp5-plasmid or Vector treated Acp5−/− PMLFs (c, p = 0.0138) and ACP5-plasmid or Vector treated PHLFs (d, p = 0.0003). e Representative results for coimmunostaining of Acp5 and β-catenin in PMLFs from WT and Acp5−/− PMLFs following TGF-β1 induction (p = 0.0127). The nuclei were stained blue by DAPI, and the images were taken under original magnification ×400. f Western blot analysis of the levels of β-catenin in cytoplasm (p < 0.0001) and nuclear (p = 0.0001). g Normalized luciferase activities of TOP-Flash over FOP-Flash relative renilla luciferase units (RLU) in PMLFs (Vector treated versus Acp5-plasmid treated: p = 0.0064, Vector treated with TGF-β1 versus Acp5-plasmid treated with TGF-β1: p = 0.0094). The data are represented as the mean ± SEM of three independent experiments. Two-sided Student’s t-test (a–b, d–g) and two-sided unpaired Student’s t-test with Welch’s correction (c) were applied. *p < 0.05; **p < 0.01; ***p < 0.001. Source data are provided as a Source Data file.