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. 2022 Jan 10;13:59. doi: 10.1038/s41467-021-27686-7

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© The Author(s) 2022

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Fig. 5 — a Alignment of several archaeal and eukaryotic hinge loops, highlighted in orange. The conserved tyrosine is highlighted in green. b Superimposition of the HsTOP1 and CsTop1 CAP domains. The hydrophobic interaction between the conserved leucine from the loop and one conserved hydrophobic pocket in the CAP domain is shown. The coloring scheme is the same as in Fig. 1. c Structural superimposition of the open and closed conformations of CsTOP1. The motion of the hinge allowing the interaction between one conserved tyrosine and DNA is shown. d Relaxation of negatively supercoiled DNA by WT and hinge mutated CsTOP1. The kinetics of relaxation is shown for the three constructs. The experiment was done twice independently.