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. 2022 Jan 10;13(1):41. doi: 10.1038/s41419-021-04485-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Western blot and B real-time PCR analysis of NK1R expression in NSCLC cell lines. C FACS analysis of NK1R expression on cellular surface membrane. HCC827 cell lysate was used as a negative control. D Western blot analysis of hHK-1 induced ERK1/2 and Akt phosphorylation in NSCLC cell lines. E Aprepitant reduced the phosphorylation of ERK1/2 and AKT triggered by hHK-1. Total ERK1/2 and Akt were used as a loading control. The effect of NK1R activation by 1 μM hHK-1 on F cell colony formation (colonies larger than 0.1 mm in diameter were counted), G cell growth curve, H transwell cell migration, and I E-cadherin/vimentin, MMP2/14 protein level. The densitometric analysis of each band was calculated with Image J software. Data were shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.