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. 2022 Jan 10;13(1):41. doi: 10.1038/s41419-021-04485-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A549 and NCI-H1944 cells were transfected with control or shNK1R plasmid. A, B The effect of NK1R knockdown on NSCLC tumor growth in nude mice. C Western blot and D IHC analysis of NK1R and Ki67 protein level in xenograft tumor samples. Scar bar, 50 μm. The IHC staining and densitometric analysis of each band were calculated with Image J software. Data were shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.