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. 2022 Jan 10;13(1):41. doi: 10.1038/s41419-021-04485-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Heatmap showing the differential NK1R expression and EGFR signaling activity in GSE2514 (NK1R^high, n = 6; NK1R^low, n = 6) and GSE130779 (NK1R^high, n = 2; NK1R^low, n = 2). B mRNA expression level of NK1R was plotted against EGFR in patient samples from GSE2514 and GSE130779. C IHC staining of NK1R and EGFR protein expression in tissue microarrays of 30 human lung adenocarcinoma patients (upper panel). IHC staining intensity of NK1R was plotted against EGFR (down panel). Scale bar, 200 μm. D Western blot and E real-time PCR analysis of NK1R and EGFR expression in NSCLC cells transfected by pLenti-EGFP-Puro-CMV-NK1R plasmid (NK1R overexpression, ov-NK1R) or by shRNA targeting NK1R (shNK1R). F IHC staining (scale bar, 50 μm), G Western blot and H real-time PCR analysis of NK1R and EGFR protein expression in the tissue samples of nude mice xenograft models. I mRNA expression level of NK1R was plotted against EGFR in tissue samples of nude mice xenograft models. The IHC staining and densitometric analysis of each band were calculated with Image J software. Data were shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.