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. 2022 Jan 10;13:78. doi: 10.1038/s41467-021-27764-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — SGL-CD1b tetramers were incorporated into a multi-parameter flow cytometry assay to measure co-receptor expression by SGL-specific T cells. a The tetramer-positive gate was defined by a dual tetramer staining with electron coupled dye (ECD) and allophycocyanin (APC)) and ‘Fluorescence Minus One’ (FMO) negative control (left and center) and a positive control using SGL-specific T cell line (A05) diluted in donor PBMC (right). b Representative staining from a South African adolescent blood donor. c The co-receptor expression of SGL-CD1b tetramer-positive T cells in the blood was quantified using cryopreserved PBMC obtained from three groups of healthy participants: U.S. controls at low risk for M.tb exposure (n = 5), South African adolescents with latent tuberculosis (IGRA-positive, n = 5), and South African adolescents without latent tuberculosis (IGRA-negative, n = 5). Boxplots depict the minimum and maximum as the smallest and largest number of the dataset, excluding outliers, the median and interquartile range tetramer-positive cells (gray) within each co-receptor group (double positive (DP), CD4, CD8, and double negative (DN), expressed as a percent of total tetramer-positive cells. Each dot represents the percent of one individual, and solid lines connect each individual. The percent of cells in each group was compared to that present in total CD3+ T cells (white). (Two-sided Wilcoxon signed-rank test with Bonferroni Correction, ** = 0.003 or 0.004, n = 15). d Boxplots depict the minimum and maximum as the smallest and largest number of the dataset, excluding outliers, the median and interquartile range of mean fluorescence intensity (MFI) of SGL-CD1b tetramer-positive cells in each co-receptor group. Each dot represents the MFI of one sample, and solid lines connect each individual. MFI was compared between groups (Two-sided Friedman test with post hoc Dunn test, ***p < 0.0001, ** = 0.003, n = 15). e Boxplots depict the minimum and maximum as the smallest and largest number of the dataset, excluding outliers, the median and interquartile range of mean fluorescence intensity (MFI) of CD3 among tetramer-positive cells in each co-receptor group. Each dot represents the MFI of one sample, and solid lines connect each individual. MFI was compared between groups (Two-sided Friedman test with post hoc Dunn test, *p = 0.05, n = 15).