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. 2022 Jan 10;13:78. doi: 10.1038/s41467-021-27764-w

Table 1.

TCRs expressed by SGL-specific T Cell Lines.

Lines TCR Variable CDR3 Joining
A01 ɑ TRAV8-6 CAVKAGYSSASKIIF TRAJ3
β TRBV12-4 CASKGKQGPEQFF TRBJ2-1
A05 ɑ TRAV13-2 CAEVLGGTSYGKLTF TRAJ52
β TRBV20-1 CSASGRRGYGYTF TRBJ1-2

Clonality was determined by staining with antibodies targeting human TCR-β variable genes (IOTest BetaMark Kit, Beckman Coulter) or high-throughput sequencing (ImmunoSEQ, Adaptive Biotechnologies). The full-length TCR sequence was determined by template-switched PCR after sorting tetramer-positive T cells. The T cell receptor gene usage and CDR3 region amino acid sequence is summarized here. Variable and joining gene segment names were assigned using the International ImMunoGeneTics (IMGT) Database.