Table 1.
Lines | TCR | Variable | CDR3 | Joining |
---|---|---|---|---|
A01 | ɑ | TRAV8-6 | CAVKAGYSSASKIIF | TRAJ3 |
β | TRBV12-4 | CASKGKQGPEQFF | TRBJ2-1 | |
A05 | ɑ | TRAV13-2 | CAEVLGGTSYGKLTF | TRAJ52 |
β | TRBV20-1 | CSASGRRGYGYTF | TRBJ1-2 |
Clonality was determined by staining with antibodies targeting human TCR-β variable genes (IOTest BetaMark Kit, Beckman Coulter) or high-throughput sequencing (ImmunoSEQ, Adaptive Biotechnologies). The full-length TCR sequence was determined by template-switched PCR after sorting tetramer-positive T cells. The T cell receptor gene usage and CDR3 region amino acid sequence is summarized here. Variable and joining gene segment names were assigned using the International ImMunoGeneTics (IMGT) Database.