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. 2022 Jan 10;8:6. doi: 10.1038/s41420-021-00803-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A H&E and IHC staining (pan-CK, CEA, and CDX-2) showed that the successfully cultured SRCC (G06) organoids and primary tumors are consistent in histological characteristics. Scale bars, 50 μm. B H&E and IHC staining (pan-CK, CEA, and CDX-2) showed that non-SRCC (G04) organoids were consistent with the primary tumor but different from SRCC, Scale bars, 50 μm. C Immunofluorescence shows that SRCC (G06 and G20) and non-SRCC (G08 and G16) have similar characteristics in the expression of EpCAM (Green) and CD133 (Red). Scale bars, 50 μm.