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. 2022 Jan 10;13:69. doi: 10.1038/s41467-021-27734-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Western blots of C/EBPB and Actin in cell lysates extracted from knockdown control (shCtrl) and two independent C/ebpb knockdown adapted E0771 cells. Three C/EBPB isoforms, LAP1, LAP2, and LIP are marked in the blots. Experiment was independently repeated twice. b The changes of tumorsphere formation upon knockdown of C/ebpb on adapted E0771 cells. Data are represented as mean ± SEM of 3 replicates. P_ctrl/sh1 < 0.0001, P_ctrl/sh2 = 0.0006. c Proliferation assay of control and C/ebpb knockdown adapted E0771 cells (n = 6 replicates). For each time point, data are represented as mean ± SEM. d, e The changes of fatty acid (d) and glucose (e) oxidation upon C/ebpb knockdown in adapted E0771 cells. The oxidation data are normalized to cell protein content (n = 12, fatty acid oxidation: P_ctrl/sh1 = 0.0028, P_ctrl/sh2 < 0.0001. Glucose oxidation: P_ctrl/sh1 = 0.0097, P_ctrl/sh2 = 0.0165). f Tumor-free survival curves of chow diet and HFD-fed mice orthotopically implanted with E0771 knockdown control and C/ebpb knockdown cells. (Chow: shCtrl n = 13, sh₁CEBPB n = 7; HFD: shCtrl n = 9, sh₁CEBPB n = 6, sh₂CEBPB n = 7; P_HFDctrl/sh1 = 0.0004, P_HFDctrl/sh2 = 0.0012, P_{chowctrl/HFDctrl} < 0.0001). g Western blots against C/EBPB in cell lysates extracted from control, LAP2 and LIP overexpressed MDA-MB-231 PA-adapted cell line. Actin was used for the normalization. Experiment was independently repeated three times. h, i The changes of tumorsphere formation upon the overexpression of C/EBPB LAP2 and LIP isoforms on adapted MDA-MB-231 (h) (n = 3, P_ctrl/LAP2 = 0.0299, P_ctrl/LIP = 0.0625) and HCC1806 (i) (n = 3, P_ctrl/LAP2 = 0.016, P_ctrl/LIP = 0.2524) cells. j, k Proliferation assay of control, LAP2 and LIP overexpressed adapted MDA-MB-231 (j) and HCC1806 (k) cells. For each time point, data are represented as mean ± SEM of 5 replicates and 4 replicates for HCC1806 LAP2 OE. l, m The changes of tumorsphere formation upon the overexpression C/EBPB LAP2 and LIP isoforms on parental HCC1806 (l) (n = 3 P_ctrl/LAP2 = 0.1836, P_ctrl/LIP = 0.0397) and MDA-MB-231 (m) (n = 6, P_ctrl/LAP2 = 0.2611, P_ctrl/LIP = 0.6529) cells. For b, d, e, h, i, l, and m, statistical significance determined with unpaired, two-tailed Student’s t-test. For f, Log-rank (Mantel–Cox) test was used for statistical testing. (NS, P value >0.05; *P value <0.05; **P value <0.01; ***P value <0.001). Source data are provided as a Source data file.