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. 2022 Jan 10;12:452. doi: 10.1038/s41598-021-04098-7

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The absence of CCR2 inhibits the accumulation of immature macrophages in the colon of IL10RA-deficient mice. (A) Representative plots showing the distribution of P1 (Ly6C⁺MHCII^-), P2 (Ly6C⁺MHCII⁺) and P3/P4 (Ly6C^-MHCII⁺) CD11b⁺CD64⁺ lamina propria macrophages as determined by flow cytometry in animals of the indicated genotype. (B) Percent and (C) Absolute number of LP macrophages in groups of WT (n = 5), CCR2KO (n = 6), IL10RAKO (n = 12) and DKO (n = 15) mice determined by flow cytometry as in (A). Please note that the y-axis on the graphs in (C) are log scale.