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. 2022 Jan 10;13(1):32. doi: 10.1038/s41419-021-04345-9

Fig. 1. Characterization of exosomes and the co-culture of RBPJ-overexpressed macrophage-derived exosomes inhibited the proliferation and induced the apoptosis of hepatocellular carcinoma (HCC) cells.

Fig. 1

A Exosomes isolated from WT THP-1 derived macrophages (WT Mφ-Exo) and RBPJ-overexpressed macrophages (RBPJ+/+ Mφ-Exo) imaged by transmission electron microscopy (TEM) are approximately 100 nm in size. Scale bar = 100 nm. B Levels of exosome markers CD63, Alix, TSG101 and HSP70 in WT or RBPJ+/+ Mφ-Exo and WT or RBPJ+/+ Mφ (Cell) were determined by Western blotting. C, D Cell proliferation in HCC cell lines SMMC-7721 and HepG2 treated with exosomes derived from RBPJ-overexpressed macrophages was assessed by a CCK-8 assay. E Flow cytometry is performed to indicate cell apoptosis. The percentage of apoptosis was then measured. All experiments were performed three times. **P < 0.01, as indicated.