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. 2022 Jan 10;13(1):32. doi: 10.1038/s41419-021-04345-9

Fig. 5. hsa_circ_0004658 acts as a sponge for miR-499b-5p.

Fig. 5

A Putative complementary sites within miR-499b-5p and hsa_circ_0004658 were predicted by bioinformatics analysis. B, C Dual-luciferase reporter assays demonstrate that miR-499b-5p is a direct target of hsa_circ_0004658 in SMMC-7721 B and HepG2 C cells (*P < 0.05, **p < 0.01). D The Ago2 RIP showed that Ago2 significantly enriched hsa_circ_0004658 and miR-499b-5p (**p < 0.01). E The level of miR-499b-5p was determined by RT-PCR in SMMC-7721 and HepG2 cells with control vector or circRNA overexpression plasmid, si-control or si-circRNA. **, ##P < 0.01, as indicated. * vs. pcDNA3.1, # vs. si-control. F The expression level of hsa_circ_0004658 in 50 hepatocellular carcinoma (HCC) tissues and matched para-carcinoma normal tissues was determined by qRT-PCR. G The expression level of miR-499b-5p in the above tissues was determined by qRT-PCR. H The expression levels of miR-499b-5p negatively correlated with hsa_circ_0004658 in HCC tissues.