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. 2022 Jan 10;5:5. doi: 10.1038/s42003-021-02945-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Airyscan microscopy images of acetylated-α-tubulin, (green) and total microtubules (magenta) in control and JIP3 KO i³Neurons (day 13) respectively. Scale bars, 5 μm. c Fraction of acetylated and non-acetylated microtubules in JIP3 KO i³Neurons (pooled data from 2 experiment with ≥19 swellings analyzed per experiment). d, e Airyscan microscopy images of neurites from both control (d) and JIP3 KO i³Neurons (day 13) (e) consist of parallel microtubule bundles that are either detyrosinated (green) or tyrosinated (magenta) in control and JIP3 KO i³Neurons, respectively. Scale bars, 5 μm. f Fraction of detyrosinated and tyrosinated microtubules in JIP3 KO i³Neurons (pooled from three independent experiments with ≥18 swellings analyzed per experiment).