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. 2022 Jan 10;8:1. doi: 10.1038/s41420-021-00796-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Western blotting of APP and the mitochondrial marker HSP60 in whole-cell extracts and mitochondrial fractions. Full-length APP and CTFs detected with APP-CTF antibody. HSP60 and Lamin A/C antibodies were used as loading controls for whole-cell extraction and mitochondrial fractions, respectively. B Quantification of APP, APP-CTFs, and HSP60 in total extracts and mitochondrial fractions. C Immunostaining of WT- and AD-iNSCs using APP-CTF and HSP60 antibodies. Nuclei were stained with DAPI. Scale bars, 10 µm. D Quantification of the colocalization area using ImageJ in WT- and AD-iNSCs. E Western blotting of OXPHOS in vehicle- or γ-secretase inhibitor-treated WT- and AD-iNSCs using OXPHOS cocktail antibodies. F Quantification of the levels of NDUFB8, COX-II, SDHB, UQCRC2, and ATP5A in WT- and AD-iNSCs-treated vehicles (−) or γ-secretase inhibitor. Statistical analysis was performed by Student’s t-test. n.s not significant, *P < 0.05, **P < 0.01, and ***P < 0.001. The results are presented as the means ± SD.