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. 2022 Jan 10;13:14. doi: 10.1038/s41467-021-27701-x

Fig. 5. MNDA is required for IRF7 induction in pDCs.

Fig. 5

a–d Quantitative PCR analysis of MNDA (a), IRF7 (b), IRF3 (c) or IRF5 (d) mRNA in CAL-1 cells expressing control or MNDA shRNA and stimulated with IFNα (1000 U/ml) for 16 h. e Anti-MNDA immunoblot (IB) of MNDA immunoprecipitated (IP) from cells treated with control or MNDA shRNA, showing effect of MNDA shRNA on MNDA protein expression. HC, antibody heavy chain. Immunoblot is representative of three experiments. All data are mean ± SD of triplicate samples and are representative of three independent experiments; two tailed unpaired Students t test; *p < 0.05 indicates significance compared to respective groups; ns indicates not significant.