Figure 2.

The characteristic and pathways of macrophages involved in murine renal crystal formation. (A) Principal component analysis (PCA) plot of the separation between the group of wild-type renal macrophages treated with GOX injection for 6 days (the wild-type group) and the group of op/op (CSF-1-deficient) renal macrophage treated with glyoxylate (GOX) injection for 6 days (the op/op group); (B) volcano plot of the differentially expressed genes (DEGs) between the wild-type group and the op/op group (P<0.05 and |log_FC| >1); (C) the enriched biological process terms of the DEGs as identified via Gene Ontology (GO) functional annotation; (D) the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway terms of the DEGs; (E) gene set enrichment analysis (GSEA) was performed to identify significantly enriched pathways.