Skip to main content
. 2021 Dec 4;298(1):101460. doi: 10.1016/j.jbc.2021.101460

Figure 5.

Figure 5

Conversion of the polar cluster to idealized hydrophobic residues increases thermal stability but affects folding cooperativity.A, Far-UV CD spectra of WT FtsLB (blue) compared with the 4×-mutant (red) at 4 °C. B, representative temperature melting curves comparing WT FtsLB (blue) to the 4×-mutant (red). The WT melting curve displays a sigmoidal transition centered around 40 °C. In contrast, the 4×-mutant displays a curve that is shifted to the right but lacks a significant transition, possibly indicating a loss of cooperativity of unfolding. CD scans were monitored at 224 nm from 4 to 89 °C. Replica melting curves are included in Fig. S4.