Fig. 8.

Lean Zucker rats on control diet (controls) compared to 8-week high-fat high-fructose diet-fed Zucker fatty rats (NASH) receiving bosentan or placebo treatment.

(A) Serum ALT and AST levels. Statistical comparison by two-way ANOVA and post hoc Scheffé. n.s., not significant. (B) Liver histological liver sections (H&E and Masson’s trichrome stains [TM], original magnification 10x). (C) Flow-pressure curves of the transhepatic pressure gradient, analysed using the generalised estimating equation model. Significances between controls and NASH are indicated by black signs. Significances between NASH with placebo or bosentan are indicated by purple signs. ∗∗∗p <0.001. ALT, alanine aminotransferase; AST, aspartate aminotransferase; NASH, non-alcoholic steatohepatitis.