Figure 2.

MLL1 physically associates with SETBP1 and SETBP1(D/N)

(A) Western blotting analysis of M2 immunoprecipitates prepared from nuclear extract of HEK293T cells transiently transfected with empty pMYs vector or pMYs construct expressing 3xFLAG-tagged Setbp1 or Setbp1(D/N) using indicated specific antibodies.

(B) Western blotting analysis of immunoprecipitates prepared with an MLL1-N-specific antibody from the same nuclear extracts as in (A) using the indicated specific antibodies.

(C) Western blotting analysis of M2 immunoprecipitates similarly prepared as in (A) using additional specific antibodies.

(D) Western blotting analysis of M2 and IgG immunoprecipitates prepared from myeloid progenitors immortalized by 3xFLAG-tagged Setbp1 or Setbp1(D/N) using indicated specific antibodies.

(E) Immunofluorescence staining of Setbp1-immortalized myeloid progenitors using a SETBP1-specific antibody together with either an MLL1-N-specific or MLL1-C-specific antibody. Nuclei were counterstained with DAPI. Scale bar, 10 μm.

(F) Same immunofluorescence staining as in (D) for Setbp1(D/N)-immortalized myeloid progenitors. Scale bar, 10 μm.