Figure 3.

Both MLL1-N1 and MLL1-C directly interact with SETBP1 and SETBP1(D/N)

(A) Schematic representation of domain structure of full-length MLL1 protein and the different 3xHA-tagged mouse MLL1 fragments used for co-immunoprecipitation studies to test possible direct interactions with 3xFLAG-tagged SETBP1 and SETBP1(D/N). Results of the co-immunoprecipitation studies shown in (B) and (C) are also summarized, with “+” indicating interaction detected with both SETBP1 and SETBP1(D/N) and “-” indicating no interaction detected.

(B) Co-immunoprecipitation experiments were performed using either anti-FLAG M2 antibody (left panels) or anti-HA antibody (right panels) on mixtures of SETBP1 with different MLL1 fragments. Inputs and immunoprecipitates were subsequently analyzed by western blotting analysis using anti-SETBP1, M2, or anti-HA antibody. SETBP1 protein and all MLL1 fragments were synthesized by in vitro transcription and translation in wheat germ extract.

(C) Same co-immunoprecipitation experiments as in (B) to detect possible direct interaction between SETBP1(D/N) and MLL1 fragments.