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. 2022 Jan 4;16:100427. doi: 10.1016/j.ynstr.2022.100427

Fig. 3.

Fig. 3

c-Fos and 5-HT immunolabeling in the dorsal raphe (DR) of the male vole brains. No group differences were found in the total number of c-Fos-labeled cells (A) or 5-HT-labeled cells (B) in the DR. Compared to the males that received immobilization (IMO) stress alone (Alone), stressed males with their partner in the cage (Partner) had a significantly lower percentage of c-Fos expressing cells co-labeled for 5-HT (C), as well as a lower percentage of 5-HT expressing cells co-labeled for c-Fos (D). Bars indicate mean ± SEM. * represents p < 0.05; ** represents p < 0.01. Panel E shows representative images of immunostaining of c-Fos, 5-HT, and double-labeled c-Fos/5-HT cells in the DR of the Alone (left) and Partner (right) males. White arrow indicates c-Fos labeled cells; black arrow indicates 5-HT labeled cells, and black arrowhead indicates cells double-labeled for c-Fos and 5-HT. Aq, cerebral aqueduct. Scale bars = 20 μm.